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MI3071 - Molecular Analysis of Tropical Microbes

Credit points: 3
Year: 2021
Student Contribution Band: Band
Administered by: College of Public Health, Medical & Vet Sciences

The subject will be available to BBiomedSc, BMedLabSc students only or by permission of the subject coordinator

This is a vocationally oriented subject aimed at providing practical training for students and future professionals with a microbial research and/or diagnostic focus. The subject introduces the molecular techniques with respect to microbial epidemiology and analysis. It covers both underlying theory and practical application. Areas that the student will be exposed to include quality assurance and measures of error, pipette maintenance, genetic techniques such as nucleic acid extraction, PCR and cloning and computer assisted analytical techniques such as primer design, sequence and SNP analysis, whole genome and next generation sequencing, database searches, testing the validity and robustness of phylogenetic data and identification of markers of microbial pathogenicity and virulence.

Learning Outcomes

  • Apply the provided theoretical knowledge to practical applications;
  • Explain causes of erroneous results and offer solutions;
  • Integrate tools for microbial epidemiology, databases and tree analyses to make hypotheses based on problem data;
  • Demonstrate a working knowledge of sequence databases and appropriate tools for interrogating and analysing these databases;
  • contribute in teams to identify the basic components of the various molecular kits, describe the function of these components and successfully utilise these kits.

Subject Assessment

  • Written > Test/Quiz 1 - (50%) - Individual
  • Performance/Practice/Product > Scenario-based learning activity - (50%) - Individual.

Special Assessment Requirements

Participate and complete any required assessment in at least 90% of the practical classes. Achieve an overall mark of >50%.

Students should enter the subject with an understanding of genomic structure, DNA replication mechanisms and microbial diversity
Prerequisites: (3 CP OF LEVEL 2 MI) AND (3 CP OF LEVEL 2 BC)


Townsville, Study Period 7, Intensive
Census Date 08-Jul-2021
Face to face teaching 21-Jun-2021 to 02-Jul-2021
Coordinator: Dr Jenny Elliman
Lecturers: Dr Jenny Elliman, Professor Bruce Gummow, Dr Sarah-Jane Wilson, Assoc. Professor Paul Horwood.
Workload expectations:

The student workload for this 3 credit point subject is approximately 130 hours.

  • 25 hours lectures (didactic or interactive) - 2.5 hours per AM session over 10 days
  • 25 hours practicals - 5 hours per day during Week 1 (Mon-Fri)
  • 25 hours workshops - 5 hours per day during Week 2 (Mon-Fri)
  • assessment and self-directed study

Note: Minor variations might occur due to the continuous Subject quality improvement process, and in case of minor variation(s) in assessment details, the Subject Outline represents the latest official information.